Tracing life at the dry limit using phospholipid fatty acids – does sampling matter?
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| Title: | Main Title: Tracing life at the dry limit using phospholipid fatty acids – does sampling matter? |
| Description: | Abstract: Phospholipid fatty acids (PLFAs) have been used to trace bacterial life in extremely carbon-poor soils of the hyperarid Atacama Desert. However, the low abundances of bacteria and, thus, PLFAs increases the risk of contamination by exogenous PLFAs. Here, we assess whether field sampling strategies (super-clean, clean, and regular sampling protocols) have an effect on PLFA diversity and abundance in hyperarid Atacama soils or whether laboratory processing or true environmental heterogeneity control PLFA inventories. Our results show no exogenous PLFA contribution during sample processing in the lab and statistical analyses (ANOVA, Kruskal-Wallis) reveal that PLFA abundances do not differ significantly between replicate samples (n=3) taken with the three different sampling strategies. Rather than sampling strategy, our results show that PLFA abundances in the investigated soil replicates rather reflect true environmental heterogeneity of primarily bacterial biomass (in the absence of indigenous fungi), potentially related to small-scale physicochemical differences. |
Responsible Party
| Creators: | Stephanie Kusch (Author), Andrea Jaeschke (Author), Ramona Mörchen (Author), Janet Rethemeyer (Author) |
| Funding Reference: | Deutsche Forschungsgemeinschaft (DFG): CRC 1211: Earth - Evolution at the Dry Limit |
| Publisher: | Elsevier Science, Amsterdam, The Netherlands |
| Publication Year: | 2019 |
Topic
| CRC1211 Topic: | Biology |
| Related Subprojects: | D4, B5 |
| Subjects: | Keywords: Biogeochemistry of Soils, Organic Geochemistry, Soil Sciences |
| Geogr. Information Topic: | Biota |
File Details
| Filename: | Kusch2020.pdf |
| Data Type: | Text - Article |
| File Size: | 1.2 MB |
| Date: | Available: 08.11.2019 |
| Mime Type: | application/pdf |
| Data Format: | |
| Language: | English |
| Status: | Completed |
Constraints
| Download Permission: | Only Project Members |
| General Access and Use Conditions: | According to the CRC1211DB data policy agreement. |
| Access Limitations: | According to the CRC1211DB data policy agreement. |
| Licence: | None |
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Specific Information - Publication
| Publication Status: | In Print |
| Review Status: | Peer reviewed |
| Publication Type: | Article |
| Article Type: | Journal |
| Source: | Soil Biology and Biochemistry |
| Volume: | 141 |
| Number of Pages: | 5 (1 - 5) |
Metadata Details
| Metadata Creator: | Andrea Jaeschke |
| Metadata Created: | 22.11.2019 |
| Metadata Last Updated: | 22.11.2019 |
| Subproject: | D4 |
| Funding Phase: | 1 |
| Metadata Language: | English |
| Metadata Version: | V50 |
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| Page Visits: | 404 |
| Metadata Downloads: | 0 |
| Dataset Downloads: | 10 |
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