The Protistan Microbiome of Grassland Soil: Diversity in the Mesoscale
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Citation
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| Title: | Main Title: The Protistan Microbiome of Grassland Soil: Diversity in the Mesoscale |
| Description: | Abstract: Genomic data for less than one quarter of ∼1.8 million named species on earth exist in public databases like GenBank. Little information exists on the estimated one million small sized (1–100 m) heterotrophic nanoflagellates and ciliates and their taxa-area relationship. We analyzed environmental DNA from 150 geo-referenced grassland plots representing topographical and land-use ranges typical for Central Europe. High through-put barcoding allowed the identification of operational taxonomic units (OTUs) at species level, with high pairwise identity to reference sequences (≥99.7%), but also the identification of sequences at the genus (≥97%) and class (≥80%) taxonomic level. Species richness analyses revealed, on average, 100 genus level OTUs (332 unique individual read (UIR) and 56 class level OTUs per gram of soil sample in the mesoscale (1–1 000 km). Database shortfalls were highlighted by increased uncertain taxonomic lineages at lower resolution (≥80% sequence identity). No single barcode occurred ubiquitously across all sites. Taxa-area relationships indicated that OTUs spread over the entire mesoscale were more similar than in the local scale and increased land-use (fertilization, mowing and grazing) promoted taxa-area separation. Only a small fraction of sequences strictly matched reference library sequences, suggesting a large protistan “dark matter” in soil which warrants further research. |
Responsible Party
| Creators: | Paul Venter (Author), Frank Nitsche (Author), Anne Domonell (Author), Peter Heger (Author), Hartmut Arndt (Author) |
| Publisher: | Esevier |
| Publication Year: | 2019 |
Topic
| CRC1211 Topic: | Biology |
| Related Subproject: | B3 |
| Subject: | Keyword: Biogeography |
File Details
| Filename: | Venter2017.pdf |
| Data Type: | Text - Article |
| File Size: | 2.9 MB |
| Date: | Available: 30.03.2017 |
| Mime Type: | application/pdf |
| Data Format: | |
| Language: | English |
| Status: | Completed |
Constraints
| Download Permission: | Free |
| General Access and Use Conditions: | According to the CRC1211DB data policy agreement. |
| Access Limitations: | According to the CRC1211DB data policy agreement. |
| Licence: | None |
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Specific Information - Publication
| Publication Status: | Accepted |
| Review Status: | Not peer reviewed |
| Publication Type: | Article |
| Article Type: | Journal |
| Source: | Protist |
| Source Website: | http://www.elsevier.de/protis |
| Volume: | 168 |
| Number of Pages: | 109 (546 - 654) |
Metadata Details
| Metadata Creator: | Frank Nitsche |
| Metadata Created: | 17.06.2019 |
| Metadata Last Updated: | 17.06.2019 |
| Subproject: | B3 |
| Funding Phase: | 1 |
| Metadata Language: | English |
| Metadata Version: | V50 |
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Dataset Statistics
| Page Visits: | 472 |
| Metadata Downloads: | 0 |
| Dataset Downloads: | 3 |
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